Genotyping of Human Papillomavirus (HPV) in precancerous and cancerous lesions of the cervix in Bamako

Abstract

Introduction: The human papilloma virus high risk (HR- HPV) is responsible of 99% cases of cervix cancer. This study was initiated to determine the types of HPV associated with precancerous and cancerous lesions of the cervix in Bamako. Material de methods: We did cross sectional prospective study form January 2016 to April 2017. Study sites was in one hand the department of anatomy and cytology pathologies of university hospital of point G (Bamako, Mali) were the histological examination of the fragments of biopsies and operative parts, coming from the screening centers, was carried out and the other hand, the molecular biology research center "Pietro Annigoni (CERBA/LABIOGENE)" of Ouagadougou (Burkina Faso) were the molecular biologic study has been realized. We did the genotyping of human papilloma virus. We searched 14 genotypes of high risk of human papilloma virus (HR-HPV) with the PCR technic at real time in 192 cervix lesions. The data analyzed on the SPSS 18.0. Results: Only 43.2% (83/192 cases) have positive test. We obtained 13 genotypes HR-HPV and the most frequent was HPV18 (16.3%), HPV45 (16.3%). The unique and multiple infections was respectively 77.1 and 22.9%. The woman HPV positive was multiparous in 30.9%. Conclusion: The most common histological type was moderate intraepithelial neoplasia (CIN2) with 42.2%. the molecular approach was indispensable in the arsenal technic of detection.