Antibiotic resistance profile of enterobacteria isolated from urinary samples at Hospital Mali

Abstract

The purpose of this study was to determine the bacteriological and antibiotic resistance profile of enterobacteria isolated in urinary tract infections at the Mali Hospital. Conventional methods of isolation and identification of bacteriology have been used. The following media and galleries were used for identification and susceptibility testing: Uriselect 4, urea-indole, Mueller Hinton, Api 20E, Api 20NE. The antibiotic susceptibility tests and the interpretation were carried out respectively by Kirby-Bauer's method (diffusion of the disks in agar medium) according to the recommendations of the antibiotic sensitivity test committee of the French Society of Microbiology. The sex-ratio H / F was 0.8. The average age was 36 years old. 30.0% of patients were older than 50 years. The prevalence of positive samples was 18.5%. Inpatients accounted for 63.2% and came from internal medicine (50.2%) and surgery (35.1%). Only 2.0% of urinary tract infections were found in the age group from 0 to 10 years. Escherichia coli was the most isolated organism (42.9%) followed by Klebsiella pneumoniae (16.3%). 70.0% of the Enterobacteriaceae identified were resistant to amoxicillin + clavulanic acid and 12.5% were producing ESBL. No strain were resistant to carbapenem. The prevalence of Enterobacteriaceae, an extremely high level of resistance to amoxicillin + clavulanic acid and a predominance of hospitalized patients were found. The present study has highlighted the increasing evolution of bacterial resistance to antibiotics, that requires adequate measures.