The bacteriological quality of the wters of consumption (drillings and Well) of three circle of the region of Koulikoro, Mali Objectifs : Contrôler la qualité bactériologique des eaux souterraines à usage alimentaire " consommation " de la région de Kouli

Abstract

Objective: Bacteriological Control quality of groundwater intended for the consumption in Koulikoro region in Mali in order to improve the health of the local population.

Materiel and Methods: For our analyzes, we used the following materials: 1 mL pipette, petri dish, thermostatic bath, thermometer, pH meter and electronic balance. Our method was based on the qualitative and quantitative research of thermo-tolerant coliforms and Escherichia coli. The culture media used were as follows: "Violet Rouge Bililé Lactose (VRBL)" to isolate coliforms and "Tryptone Bile X-Glucuronide Agar (TBX)" to isolate Escherichia Coli. After incubation, the colonies were counted to determine the conformity of the samples where we used French standards NF V08-060 for Coliforms Thermo-tolerant and NF EN ISO 16649-2 for Escherichia coli.

Results : During our study, we collected 172 samples by collecting 900 ml of water samples in 80 wells and 92 drillings in the Koulikoro area. Among our 172 samples, 75 or 43.6% were not in conformity and could be due to the non-protection of the wells and the absence of the basic hygiene rules. Well water was more contaminated with 94.7% of non-conformity than drilling waters of 5.3%. Dioila area recorded more cases of non-conformity (53.3%) followed by Kati (26.7) and Banamba (20%). Escherichia Coli and Coliforms Thermo-tolerant have been both sources of contamination of non-conformity waters